Pectinase Production by Aspergillus niger and Its Applications in Fruit Juice Clarification

Pectinases (EC 3.2.1.15) are a class of enzymes that catalyze the depolymerization or de-esterification reactions degrade pectic substances. In present study, we have isolated Aspergillus niger strain from soil samples, in India and evaluated pectinase production. The highest producing A. was further optimized with various agricultural wastes. Plackett-Burman design (PBD) Central composite ‘(CCD)’ were used to determine best parameters for maximum Pectinase activity increased 99.21 U/ml after optimizing production medium using PBD CCD statistical analysis. A positive correlation between predicted (112.65 U/ml) experimental (99.21 SD=0.005) optimum observed. Maximum produced by under submerged fermentation, utilizing orange peel, which is cost-effective, adaptable, environmentally friendly approach. partially purified showed significant application apple juice clarification ability pectin therefore colour change observed within 120 min. waste peel fermentation an economical, versatile eco-friendly process clarification.